ea0020p628 | Reproduction | ECE2009
Esponda Pedro
, Relloso Miguel
The female genital tract of the mouse was in vivo transfected using the reporter gene β-galactosidase and the liposome Lipofectamine as gene vector. All animals used were anaesthetised. DNA/Liposome complexes were injected through the infundibulum of the tubes in adult, immature and pseudopregnant females. Females that were at different stages of the ovarian cycle were also used. Transfection was analyzed using histochemical, immunological and molecular (Southern b...